SURVIVAL OF PLATELET DENSITY SUBPOPULATIONS

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Heterogeneity of Human Whole Blood Platelet Subpopulations

A quantitative high yield method utilizing isosmotic arabino-galactan (Stractan) solutions and isopycnic centrifugation was developed to isolate and to fractionate total human platelet populations into density-dependent subpopulations. Isolated platelets were free of factor VIll/von Willebrand factor and other plasma proteins. They responded to ADP, epinephrine, and collagen with a sensitivity ...

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Heterogeneity of human whole blood platelet subpopulations. I. Relationship between buoyant density, cell volume, and ultrastructure.

A quantitative high yield method utilizing isosmotic arabino-galactan (Stractan) solutions and isopycnic centrifugation was developed to isolate and to fractionate total human platelet populations into density-dependent subpopulations. Isolated platelets were free of factor VIII/von Willebrand factor and other plasma proteins. They responded to ADP, epinephrine, and collagen with a sensitivity ...

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Heterogeneity of human whole blood platelet subpopulations. III. Density-dependent differences in subcellular constituents.

Structurally intact platelet cohorts of differing densities can be isolated from normal subjects by the use of isosmolar arabinogalactan density gradients. Using platelets separated in this fashion, we have studied the density-dependent distribution of four subcellular organelles: mitochondria, lysosomes, dense bodies, and alpha granules. Mitochondria, which are not secreted during platelet rel...

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The relation of platelet density to platelet age: survival of low- and high-density 111indium-labeled platelets in baboons.

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Platelet heterogeneity: variation in coagulation complexes on platelet subpopulations.

OBJECTIVE Previous work has shown that platelets stimulated with the combination of thrombin and convulxin, a glycoprotein VI agonist, develop 2 populations with different levels of alpha-granule factor V bound to the platelet surface. To evaluate whether this phenomenon is restricted to alpha-granule components or is a feature that can be generalized to other coagulation factors, we studied th...

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ژورنال

عنوان ژورنال: British Journal of Haematology

سال: 1987

ISSN: 0007-1048

DOI: 10.1111/j.1365-2141.1987.tb04167.x